ExoFlow-ONE EV Labeling Kit for Flow Cytometry (Ruby Red)

Harness the power of flow cytometry for EV analysis Finally, you can take full advantage of the power of flow-based methods for analyzing extracellular vesicles (EVs) with SBI’s exclusive ExoFlow-ONE Gemstone dyes. By specifically labeling internal EV components with one of these proprietary, high quantum efficiency dyes, you can achieve near single-vesicle resolution* for more powerful flow cytometry and FACS studies, and uncover greater insights into EV biology. ExoFlow-ONE EV Labeling Kits: Directly detect EVs by specifically labeling internal EV components Achieve near single-vesicle visualization* with our proprietary ExoFlow-ONE dyes that deliver High quantum efficiency Negligible background signal when not bound to EVs Accurately estimate the size of labeled EVs and ensure proper run-to-run calibration with the included size standards Learn more from a single sample by multiplexing with our range of spectrally separated ExoFlow-ONE dyes The ExoFlow-ONE Ruby Red Gemstone Dye Kit comes with enough labeling reagents for 25 reactions**. Each kit also includes 1.5 mL of silica bead size standards (110 nm – 1300 nm), two of which are fluorescent. The silica beads, sourced from Apogee Flow Systems, are specifically designed to produce a refractive index and light scattering patterns similar to biological particles, enabling more accurate estimation of EV particle size. *Requires a specialized flow cytometry imager capable of high-resolution vesicle analysis (e.g. Amnis® ImageStreamX Mark II, Apogee Micro-PLUS). **Each reaction is defined as 1 µL of working dye combined with 200 – 500 µg of EV protein resuspended in 500 µL of buffer. Choose the right ExoFlow-ONE dye for your project Cat.#ExoFlow ONE DyeExcitation/Emission WavelengthRecommended Laser Line EXOF100A-1Ruby Red573/588 nm561 nm EXOF200A-1Garnet Far Red628/643 nm633 nm EXOF300A-1Emerald Green511/525 nm488 nm EXOF400A-1Sapphire Blue403/454 nm405 nm EXOF500A-1Citrine Yellow542/556 nm532 nm

ExoFlow-ONE EV Labeling Kit for Flow Cytometry, Ruby Red

Directly detect extracellular vesicles down to near single-vesicle resolution for more powerful insights from flow cytometry applications Directly detect EVs by specifically labeling internal EV components Achieve near single-vesicle visualization* with our proprietary ExoFlow-ONE dyes that deliver High quantum efficiency Negligible background signal when not bound to EVs Accurately estimate the size of labeled EVs and ensure proper run-to-run calibration with the included size standards Learn more from a single sample by multiplexing with our range of spectrally separated ExoFlow-ONE dyes