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品牌: Rockland
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
反应种属:
Goat
产品介绍
产品介绍
产品信息
荧光素标记
Rhodamine (TRITC)

宿主
Rabbit

免疫原
Goat IgG whole molecule

简单描述
Rabbit Fab Anti-Goat IgG (H&L) Antibody Rhodamine Conjugated - 805-4002

商品描述
Rabbit Fab Anti-Goat IgG (H&L) Antibody Rhodamine Conjugated - 805-4002

同种型
IgG Fab

克隆类别
Polyclonal

纯度
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).

浓度
1.0 mg/mL by UV absorbance at 280 nm

形式
Lyophilized

防腐剂
0.01% (w/v) Sodium Azide

缓冲体系
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

应用
实验应用
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

推荐用量
500 µL

反应种属
Goat

目标/特异性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).

样本类型以及上样量
500 µL

背景
别名
Rabbit Fab Anti-Goat Antibody Rhodamine Conjugation, Rabbit Fab Anti-Goat TRITC Conjugated Antibody

背景
Fab Anti-Goat IgG Rhodamine Antibody generated in rabbit detects goat IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

保存方式
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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