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Donkey Fab Anti-Goat IgG (H&L) Antibody Biotin Conjugated - 805-7602
品牌： Rockland
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Fab Anti-Goat IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
反应种属：
Goat
产品介绍
产品介绍
产品信息
荧光素标记
Biotin
宿主
Donkey
免疫原
Goat IgG whole molecule
简单描述
Donkey Fab Anti-Goat IgG (H&L) Antibody Biotin Conjugated - 805-7602
商品描述
Donkey Fab Anti-Goat IgG (H&L) Antibody Biotin Conjugated - 805-7602
同种型
IgG Fab
克隆类别
Polyclonal
纯度
Fab Anti-Goat IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
浓度
1.0 mg/mL by UV absorbance at 280 nm
形式
Lyophilized
防腐剂
0.01% (w/v) Sodium Azide
缓冲体系
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
应用
实验应用
ELISA, IHC;Fab Anti-Goat IgG Biotin Conjugated antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot
推荐用量
1.0 mL
反应种属
Goat
目标/特异性
Fab Anti-Goat IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
样本类型以及上样量
1.0 mL
背景
别名
Donkey Fab Anti-Goat IgG Biotin Conjugated Antibody, Donkey Fab Fragment Anti-Goat IgG Antibody Biotin Conjugation
背景
Fab Anti-Goat IgG Antibody generated in donkey detects goat IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
保存方式
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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