Phospho-FAK (Tyr397) (D20B1) Rabbit mAb

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7). 1.Parsons, J.T. et al. (2000) Oncogene 19, 5606-13. 2.Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8. 3.Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55. 4.Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34. 5.Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6. 6.Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63. 7.Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.