首页
rcynoFcg RI/CD64, CF (100 ug)
rcynoFcg RI/CD64, CF (100 ug)

rcynoFcg RI/CD64, CF (100 ug)

分享
品牌： R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    产品介绍
    产品信息

    Scientific Data

    Bioactivity Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein BioactivityView Larger

    When Human IgG is immobilized at 0.5 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey Fc gamma RI/CD64 (Catalog # 9239-FC) binds with an ED50 of 1-5 ng/mL.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    9239-FC

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 200 μg/mL in PBS.
    ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein, CF Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey Fc gamma  RI/CD64 that produces 50% of the optimal binding response is approximately 1-5 ng/mL.
    Source
    Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RI/CD64 protein
    Val11-Pro288, with a C-terminal 6-His tag
    Accession #
    NP_001270969
    N-terminal Sequence
    Analysis
    Val11
    Predicted Molecular Mass
    32 kDa
    SDS-PAGE
    38-46 kDa, reducing conditions
    背景
    别名
    CD64 antigen,CD64,CD64a,Fc fragment of IgG, high affinity Ia, receptor (CD64),Fc gamma RI,FCG1,Fc-gamma receptor I B2,Fc-gamma RI,Fc-gamma RIA,FcgammaRIa,FCGR1,FcgRI,FcgRIA,FCRI,FcRIA,FLJ18345,high affinity Ia, receptor for (CD64),high affinity immunoglobulin gamma Fc receptor I,IgG Fc receptor I,Fc gamma Receptor I
    背景

    Background: Fc gamma RI/CD64

    Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1, 2). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI/CD64, Fc gamma  RII/CD32, and Fc gamma  RIII/CD16. Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8 -10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1, 3). Mature cynomolgus Fc gamma RI consists of a 277 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RI shares 95%, 72%, and 66% aa sequence identity with human, mouse, and rat Fc gamma RI, respectively. It binds cynomolgus IgG subclasses 1-4 as well as human IgG 1, 3, and 4 (4, 5). It delivers an activating signal via the associated Fc R gamma accessory chain (6). Fc gamma RI is expressed constitutively on monocytes, macrophages, and monocyte-derived dendritic cells and can be induced on neutrophils, eosinophils, mast cells, and glomerular mesangial cells (1, 3). Its expression is up-regulated during bacterial infections and sepsis.

    References
    1. Chenoweth, A.M. et al. (2015) Immunol. Rev. 268:175.
    2. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
    3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
    4. Warncke, M. et al. (2012) J. Immunol. 188:4405.
    5. Nguyen, D.C. et al. (2014) Immunogenetics 66:361.
    6. van Vugt, M.J. et al. (1996) Blood 87:3593.
    Long Name
    Fc gamma Receptor I
    Entrez Gene IDs
    2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)
    Alternate Names
    CD64 antigen; CD64; CD64a; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FcgRIA; FCRI; FcRIA; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 200 μg/mL in PBS.

    参考图片

    When Human IgG is immobilized at 0.5 μg/mL, 100 μL/well,Recombinant Cynomolgus Monkey Fc gamma RI/CD64 (Catalog # 9239-FC) binds withan ED50 of
    1-5 ng/mL.

    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    9239-FC-100
    一键复制
    询价
    100ug
    选择数量
    当前规格1件起购 
    配送至
    预计2-3周送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价