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Immunohistochemical analysis of paraffin-embedded HeLa cell pellet (left, high-expressing) or HL-60 cell pellet (right, low-expressing) using FTH1 (D1D4) Rabbit mAb.
Western blot analysis of extracts from Huh7 and Hep G2 cells using xCT/SLC7A11 (D2M7A) Rabbit mAb.
Immunoprecipitation of xCT/SLC7A11 protein from A549 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is xCT/SLC7A11 (D2M7A) Rabbit mAb. Western blot analysis was performed using xCT/SLC7A11 (D2M7A) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from MEF cells treated with DEM (50 μM, 3 hr) and NRF2 (D1Z9C) XP® Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across NQO1, a known target gene of NRF2 (see additional figure containing ChIP-qPCR data).
Western blot analysis of extracts from MEF wt and U-2 OS cells, untreated (-) or treated with MG-132 #2194 (10 μM, 10 hr; +), using NRF2 (D1Z9C) XP® Rabbit mAb.
Western blot analysis of extracts from SK-MEL-5 and SK-MEL-2 cells using DMT1/SLC11A2 (D3V8G) Rabbit mAb.
Western blot analysis of extracts from HeLa and HT-29 cells using FTH1 (D1D4) Rabbit mAb.
Simple Western™ analysis of lysates (1.0 mg/mL) from HeLa cells using FTH1 (D1D4) Rabbit mAb #4393. The virtual lane view (left) shows the target (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation of FTH1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is FTH1 (D1D4) Rabbit mAb. Western blot was performed using FTH1 (D1D4) Rabbit mAb.
Western blot analysis of extracts from various cell lines using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using GPX4 Antibody.
Western blot analysis of extracts from various cell lines using NCOA4 (E8H8Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, HT-1080 and OVCAR-4 cells are low for NCOA4 expression.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from various cell lines using KEAP1 (D6B12) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from MEF cells treated with DEM (50 μM, 3 hr) and NRF2 (D1Z9C) XP® Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 8 (upper), including NQO1 (lower), a known target gene of NRF2 (see additional figure containing ChIP-qPCR data).
Immunoprecipitation of NRF2 from MEF wt cell extracts treated with MG-132 #2194 (10 μM, 10 hr) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or NRF2 (D1Z9C) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NRF2 (D1Z9C) XP® Rabbit mAb (lane 3).
Western blot analysis of extracts from human SK-MEL-5 cells, untreated (-) or treated with PNGase F (+), using DMT1/SLC11A2 (D3V8G) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using FTH1 (D1D4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb.
Western blot analysis of extracts from mouse testis using GPX4 Antibody.
Immunoprecipitation of NCOA4 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NCOA4 (E8H8Z) Rabbit mAb. Western blot analysis was performed using NCOA4 (E8H8Z) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Western blot analysis of extracts from OVCAR8 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® KEAP1 siRNA I #5285 (+) or SignalSilence® KEAP1 siRNA II #5289 (+), using KEAP1 (D6B12) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The KEAP1 (D6B12) Rabbit mAb confirms silencing of KEAP1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
Chromatin immunoprecipitations were performed with cross-linked chromatin from MEF NRF2 wild-type (left) and NRF2 knock-out (right) cells, both treated with DEM (50 μM, 3 hr), and NRF2 (D1Z9C) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse MafG intron 1 primers, SimpleChIP® Mouse NQO1 Promoter Primers #12635, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Confocal immunofluorescent analysis of wild-type NRF2 MEF cells, untreated (left) or treated with MG-132 #2194 (10 μM, 8 hr; center), and NRF2 knock-out MEF cells treated with MG-132 #2194 (10 μM, 8 hr; right), using NRF2 (D1Z9C) XP® Rabbit mAb (green pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 Phalloidin #8878 (red pseudocolor).
Immunoprecipitation of DMT1 from SK-MEL-5 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DMT1/SLC11A2 (D3V8G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DMT1/SLC11A2 (D3V8G) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using FTH1 (D1D4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb.
Confocal immunofluorescent analysis of OVCAR8 cells using KEAP1 (D6B12) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of MEF wt cells, untreated (blue) or treated with MG-132 #2194 (10uM, 4 hrs; green) using NRF2 (D1Z9C) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using FTH1 (D1D4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using FTH1 (D1D4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using FTH1 (D1D4) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa (left, high expressing) or SH-SY5Y (right, low expressing) cells using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded normal human lung using FTH1 (D1D4) Rabbit mAb.
Flow cytometric analysis of SH-SY5Y cells (blue) and HeLa cells (green) using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded normal mouse colon using FTH1 (D1D4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal mouse liver using FTH1 (D1D4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal mouse spleen using FTH1 (D1D4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal mouse testis using FTH1 (D1D4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using FTH1 (D1D4) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).