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FGF Receptor 1 (D8E4) XP ® Rabbit mAb

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FGF Receptor 1 (D8E4) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

FGF Receptor 1

来源纯化

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:100 - 1:400
Immunofluorescence (Immunocytochemistry)	1:200 - 1:800
Flow Cytometry (Fixed/Permeabilized)	1:200 - 1:800

同种型

Rabbit IgG

分子量

92 , 120, 145

研究领域

癌症,细胞生物学,

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

FGF Receptor 1 (D8E4) XP Rabbit mAb detects endogenous levels of total FGF receptor 1 protein. This antibody may slightly cross-react with overexpressed FGF receptor family members.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components, such as Crk and PLCγ (4,5). 1.Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97. 2.Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8. 3.Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89. 4.Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78. 5.Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.

研究领域

癌症,细胞生物学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #76123.

数据库链接

Entrez-Gene ID

2260

UniProt ID

P11362

参考图片

Flow cytometric analysis of A-204 cells using FGF Receptor 1 (D8E4) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from A-204 (FGFR1 positive), KG-1a (FGFR1 oncogenic partner-FGFR1 fusion), A172 (FGFR1 low), and HT-29 (FGFR1 negative) cells using FGF Receptor 1 (D8E4) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FGF Receptor 1 (D8E4) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human kidney using FGF Receptor 1 (D8E4) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FGF Receptor 1 (D8E4) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded A-204 cell pellet (left, positive) or HT-29 cell pellet (right, negative) using FGF Receptor 1 (D8E4) XP® Rabbit mAb.

Confocal immunofluorescent analysis of A204 cells (positive, left), KG-1 cells (positive, middle) and A172 cells (weak expression, right) using FGF Receptor 1 (D8E4) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

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货号：

9740S

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