Fixable Viability Stain 450 100ug

BD Horizon™ Fixable Viability Stain 450 (FVS450) is useful to discriminate viable from non-viable mammalian cells in multicolor flow cytometric applications. This violet fluorescent stain contains a dye that reacts with and covalently binds to cell surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the violet dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells. Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeablization and subsequent intracellular staining while maintaining stable FVS450 fluorescence. The BD Horizon™ Fixable Viability Stain 450 is excited by the Violet laser (with an excitation maximum of 406 nm) and has a fluorescence emission maximum at 450 nm.

BD Horizon™ Fixable Viability Stain 450 (FVS450) is useful to discriminate viable from non-viable mammalian cells in multicolor flow cytometric applications. This violet fluorescent stain contains a dye that reacts with and covalently binds to cell surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the violet dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells. Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeablization and subsequent intracellular staining while maintaining stable FVS450 fluorescence. The BD Horizon™ Fixable Viability Stain 450 is excited by the Violet laser (with an excitation maximum of 406 nm) and has a fluorescence emission maximum at 450 nm.

Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)

Live/Dead Discriminator Dyes

研发参考(4) 1. Abrams B, Diwu Z, Guryev O, et al. 3-Carboxy-6-chloro-7-hydroxycoumarin: a highly fluorescent, water-soluble violet-excitable dye for cell analysis. Anal Biochem. 2009; 386(2):262-269. (Methodology). 2. Burmeister Y, Lischke T, Dahler AC, et al. ICOS controls the pool size of effector-memory and regulatory T cells. J Immunol. 2008; 180(2):774-782. (Methodology). 3. Charles ED, Green RM, Marukian S, et al. Clonal expansion of immunoglobulin M+CD27+ B cells in HCV-associated mixed cryoglobulinemia. Blood. 2008; 111(3):1344-1356. (Methodology). 4. Perfetto SP, Chattopadhyay PK, Lamoreaux L, et al. Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry. J Immunol Methods. 2006; 313(1–2):199-208. (Methodology).