BD Cytofix/Cytoperm™ Plus Fixation/Permeabilization Solution Kit with BD GolgiPlug™

This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining. This kit also provides an alternative protein transport inhibitor, BD GolgiPlug™ containing brefeldin A. Sufficient BD GolgiPlug reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 10 ⁶ cells/ml. Components Quantity  Fixation/Permeabilization solution  125 mL  BD Perm/Wash™ buffer (10x)  100 mL  BD GolgiPlug protein transport inhibitor containing brefeldin A  1 mL

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Cytofix/Cytoperm with Golgiplug

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