

产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
FLT3

来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Tyr842 of human FLT3.

宿主
Rabbit

商品描述
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |

同种型
Rabbit IgG

分子量
160

研究领域
癌症,细胞生物学,

应用
反应种属
Human,Mouse,

目标/特异性
Specificity/Sensitivity
Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb detects endogenous levels of FLT3 only when phosphorylated at Tyr842. This antibody may cross-react with other tyrosine-phosphorylated family members.
Species Reactivity:
Human, Mouse

敏感性
Endogenous

背景
背景
FMS-related tyrosine kinase 3 (FLT3, also called FLK2) is a member of the Type III receptor tyrosine kinase family, which includes c-Kit, PDGFR, and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream substrates (3). The p85 subunit of PI3 kinase, SHP2, GRB2, and Shc have all been reported to associate with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).Tyr842 is located in the activation loop of FLT3. Mutation of Tyr842 was identified in human AML patients (8).
1.Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.
2.Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.
3.Namikawa, R. et al. (1996) Stem Cells 14, 388-95.
4.Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.
5.Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.
6.Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.
7.Mizuki, M. et al. (2000) Blood 96, 3907-14.
8.Kindler, T. et al. (2005) Blood 105, 335-340.

研究领域
癌症,细胞生物学,
翻译后修饰
phosphate

制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
2322

UniProt ID
P36888

参考图片
Western blot analysis of cell extracts from SEM cells expressing constitutively activated FLT3 using Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb (upper and middle). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. Total FLT3 (8F2) Rabbit mAb #3462 shown at the bottom.
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