Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb

FMS-related tyrosine kinase 3 (FLT3, also called FLK2) is a member of the Type III receptor tyrosine kinase family, which includes c-Kit, PDGFR, and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream substrates (3). The p85 subunit of PI3 kinase, SHP2, GRB2, and Shc have all been reported to associate with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).Tyr842 is located in the activation loop of FLT3. Mutation of Tyr842 was identified in human AML patients (8). 1.Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48. 2.Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30. 3.Namikawa, R. et al. (1996) Stem Cells 14, 388-95. 4.Beslu, N. et al. (1996) J Biol Chem 271, 20075-81. 5.Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9. 6.Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80. 7.Mizuki, M. et al. (2000) Blood 96, 3907-14. 8.Kindler, T. et al. (2005) Blood 105, 335-340.