BD Phosflow™ Fluorescent Cell Barcoding Wash Buffer

BD Phosflow™ Fluorescent Cell Barcoding Wash Buffer can be used for washing and staining cells after Fluorescent Cell Barcoding, ie, labeling cells with Cell Barcoding Dyes (either CBD450 or CBD500 dye or mixtures of both). Moreover, the Fluorescent Cell Barcoding Wash Buffer is useful for the dilution and application of fluorescent antibodies to barcoded cells as well as for the suspension, washing, and short-term storage of barcoded cells destined for flow cytometric analysis. Fluorescent Cell Barcoding Wash Buffer is formulated as a neutral pH (pH 7.4)-buffered salt solution that is supplemented with fetal bovine serum and bovine serum albumin. As such, Fluorescent Cell Barcoding Wash Buffer is designed to maintain single-cell suspensions well and to maximize fluorescence signal intensities. The Fluorescent Cell Barcoding Wash Buffer contains the metabolic inhibitor, sodium azide as a preservative.

(RUO)

Intracellular staining (flow cytometry) (Routinely Tested)

Human (QC Testing)

Fluorescent cell barcoding (FCB)

Aqueous buffered solution containing BSA, fetal bovine serum and ≤0.09% sodium azide.