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品牌： BD Pharmingen
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对比
咨询反应种属：
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
来源宿主：
Mouse BALB/c IgG1
产品介绍
产品介绍
产品信息
抗原名称
FoxP3
宿主
Mouse BALB/c IgG1
免疫原
FoxP3
简单描述
The 259D/C7 monoclonal antibody specifically recognizes human Forkhead box protein P3 (FoxP3) that is also known as Scurfin. FoxP3 is encoded by
FOXP3
(Forkhead box P3), likewise known as
IPEX
(Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked) and
JM2
, that belongs to the forkhead/winged-helix family of transcriptional regulators. FoxP3 is expressed in CD4+ regulatory T cells (Treg) and represents a specific marker for these cells. Flow cytometric analyses have shown that FoxP3 is expressed by the majority of CD4+CD25+high T cells in peripheral blood while less than half of the CD4+CD25+intermediate cell population are FoxP3 positive. Approximately 5-10% of peripheral CD4+ cells are CD4+CD25+ T regulatory cells. T regulatory cells are thought to play a critical role in the regulation of T cell-mediated immunity and to protect against autoimmunity by suppressing the proliferation and cytokine production of other T cells. In support of this hypothesis, it has been found that
Foxp3
is mutated in Scurfy (sf) mice that have defective T cell tolerance leading to an X-linked lymphoproliferative disease. The 259D/C7 antibody recognizes all currently-identified isoforms of human FoxP3 and is crossreactive with FoxP3 from Cynomolgus, Rhesus and Baboon primates.
商品描述
259D/C7
The 259D/C7 monoclonal antibody specifically recognizes human Forkhead box protein P3 (FoxP3) that is also known as Scurfin. FoxP3 is encoded by
FOXP3
(Forkhead box P3), likewise known as
IPEX
(Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked) and
JM2
, that belongs to the forkhead/winged-helix family of transcriptional regulators. FoxP3 is expressed in CD4+ regulatory T cells (Treg) and represents a specific marker for these cells. Flow cytometric analyses have shown that FoxP3 is expressed by the majority of CD4+CD25+high T cells in peripheral blood while less than half of the CD4+CD25+intermediate cell population are FoxP3 positive. Approximately 5-10% of peripheral CD4+ cells are CD4+CD25+ T regulatory cells. T regulatory cells are thought to play a critical role in the regulation of T cell-mediated immunity and to protect against autoimmunity by suppressing the proliferation and cytokine production of other T cells. In support of this hypothesis, it has been found that
Foxp3
is mutated in Scurfy (sf) mice that have defective T cell tolerance leading to an X-linked lymphoproliferative disease. The 259D/C7 antibody recognizes all currently-identified isoforms of human FoxP3 and is crossreactive with FoxP3 from Cynomolgus, Rhesus and Baboon primates.
同种型
Mouse BALB/c IgG1
克隆号
克隆 259D/C7 (RUO)
产品详情
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
目标/特异性
FoxP3
背景
别名
scurfin; IPEX; JM2; AIID; XPID; DIETER; PIDX
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Brunkow ME, Jeffery EW, Hjerrild KA, et al. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat Genet. 2001; 27(1):68-73. (Biology).
2. Giovanna Roncador et al. Analysis of Foxp3 protein expression in human CD4+CD25+ regulatory Tcells at a single cell level. Eur J Immunol. 2005; 35(Immunogen).
3. Wildin RS, Ramsdell F, Peake J, et al. X-linked neonatal diabetes mellitus, enteropathy and endocrinopathy syndrome is the human equivalent of mouse scurfy. Nat Genet. 2001; 27(1):18-20. (Biology).
参考图片
Flow cytometric analysis of FoxP3 on resting PBMC. Human PBMC were stained with FITC Mouse Anti-Human CD4 (Cat. No. 555346) and PE Mouse Anti-Human CD25 (Cat. No. 555432) simultaneously. Cells were fixed and permeabilized (see recommended assay procedure) followed by intracellular staining with Alexa 647 anti-human FoxP3 (Cat No. 560045). The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 (left panel) or FoxP3 vs CD4 (right panel). Flow cytometry was performed on a BD FACSCalibur™ System.
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