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产品介绍
产品信息
抗原名称
Foxp3
宿主
Rat IgG2b
免疫原
Mouse Foxp3 Recombinant Protein
简单描述
The MF23 monoclonal antibody specifically binds to mouse Foxp3. Foxp3 is a 50-55 kDa protein also known as Forkhead box p3, JM2, or IPEX. It is a member of the forkhead or winged helix family of transcription factors and is specifically expressed by T regulatory (Treg) cells. Foxp3 has been reported to be a key regulatory protein for Treg cell development and function. Ectopic expression of Foxp3 in conventional T cells is sufficient to induce suppressive activity, repress the production of cytokines such as IL2 and IFN-γ, and upregulate Treg cell-associated molecules such as CD25, CTLA4 and GITR. It has been found that the mutation of Foxp3 is responsible for "scurfy" mice. When overexpressed, Foxp3 leads to poor T cell proliferation and activation. Immunoblotting with MF23 antibody has confirmed it recognizes an epitope between 1-87 amino acids in the N-terminal domain.
商品描述
MF23
The MF23 monoclonal antibody specifically binds to mouse Foxp3. Foxp3 is a 50-55 kDa protein also known as Forkhead box p3, JM2, or IPEX. It is a member of the forkhead or winged helix family of transcription factors and is specifically expressed by T regulatory (Treg) cells. Foxp3 has been reported to be a key regulatory protein for Treg cell development and function. Ectopic expression of Foxp3 in conventional T cells is sufficient to induce suppressive activity, repress the production of cytokines such as IL2 and IFN-γ, and upregulate Treg cell-associated molecules such as CD25, CTLA4 and GITR. It has been found that the mutation of Foxp3 is responsible for "scurfy" mice. When overexpressed, Foxp3 leads to poor T cell proliferation and activation. Immunoblotting with MF23 antibody has confirmed it recognizes an epitope between 1-87 amino acids in the N-terminal domain.
同种型
Rat IgG2b
克隆号
克隆 MF23 (RUO)
浓度
0.2 mg/ml
产品详情
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Mouse (QC Testing)
背景
别名
Forkhead box p3; Forkhead box protein p3; JM2; Scurfin; Scurfy; Sf
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Hori S, Nomura T, Sakaguchi S. Control of regulatory T cell development by the transcription factor Foxp3. Science. 2003; 299(5609):1057-1061. (Biology).
2. Ono M, Yaguchi H, Ohkura N, et al. Foxp3 controls regulatory T-cell function by interacting with AML1/Runx1. Nature. 2007; 446(7136):685-689. (Biology).
3. Zheng Y, Rudensky AY. Foxp3 in control of the regulatory T cell lineage. Nat Immunol. 2007; 8:457-462. (Biology).
参考图片
Flow cytometric analysis of Alexa Fluor® 647 Rat anti-Mouse Foxp3 on mouse splenocytes. Balb/c mouse splenocytes were surface stained with FITC anti-mouse CD4 (clone RM4-5, Cat. No. 553047), then fixed and permeabilized using working solutions of Mouse Foxp3 Buffer Set (see recommended assay procedure, Cat. No. 560409) followed by intracellular staining with Alexa Fluor® 647 Rat anti-Mouse Foxp3 (0.03 µg/test). The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes. Flow cytometry was performed on a BD FACSCalibur™ System.
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