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FTH1 (D1D4) Rabbit mAb

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FTH1 (D1D4) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

FTH1

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FTH1 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:200 - 1:800

同种型

Rabbit IgG

分子量

研究领域

癌症,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

FTH1 (D1D4) Rabbit mAb recognizes endogenous levels of total FTH1 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Ferritin is a ubiquitous and highly conserved protein that plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form (1). The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron (2,3). It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain (FTH) and ferritin light chain (FTL), each having unique functions. FTHs catalyze the first step in iron storage, the oxidation of Fe(II), whereas FTLs promote the nucleation of ferrihydrite, enabling storage of Fe(III) (4). In addition to iron buffering, FTH also enhances thymidine biosynthesis (5). Serum ferritin levels indicate the amount of iron stored in the body and is the most sensitive test for anemia. It is markedly elevated in inflammation, malignancy, and iron overload disorders (6). Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases (7). 1.Munro, H.N. et al. (1988) Ann N Y Acad Sci 526, 113-23. 2.Theil, E.C. (2004) Annu Rev Nutr 24, 327-43. 3.Theil, E.C. et al. (2006) J Biol Inorg Chem 11, 803-10. 4.Treffry, A. et al. (1992) FEBS Lett 302, 108-12. 5.Oppenheim, E.W. et al. (2001) J Biol Chem 276, 19855-61. 6.Tarng, D.C. (2009) Nat Clin Pract Nephrol 5, 66-7. 7.Fischer, P. et al. (1997) Life Sci 60, 2273-8.

研究领域

癌症,代谢,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

2495

UniProt ID

P02794

参考图片

Immunohistochemical analysis of paraffin-embedded HeLa cell pellet (left, high-expressing) or HL-60 cell pellet (right, low-expressing) using FTH1 (D1D4) Rabbit mAb.

Western blot analysis of extracts from HeLa and HT-29 cells using FTH1 (D1D4) Rabbit mAb.

Simple Western™ analysis of lysates (1.0 mg/mL) from HeLa cells using FTH1 (D1D4) Rabbit mAb #4393. The virtual lane view (left) shows the target (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunoprecipitation of FTH1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is FTH1 (D1D4) Rabbit mAb. Western blot was performed using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal human lung using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal mouse colon using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal mouse liver using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal mouse spleen using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal mouse testis using FTH1 (D1D4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using FTH1 (D1D4) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

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货号：

4393S

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20ul

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