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产品介绍
产品介绍
产品信息
商品描述
FURA 2/AM is the cell-permeable ester form of FURA 2 ( & ), which is a fluorescent chelator (Kd = 224 nM) selective for Ca2+ over other ions and offers 30 times greater fluorescence intensity than QUIN 2 . FURA 2/AM is rapidly metabolized in the cell by cytoplasmic esterases; yielding the active dye FURA 2. FURA 2/AM can be used as a probe to determine calcium concentrations in many cellular systems, including: cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max = 362 nm, emission max = 512 nm) differ from those during high calcium conditions (excitation max = 335 nm, emission max = 505 nm).
分子量
1001.85
可溶性
Soluble in DMSO (10 mg/ml), DMF, CH3CN, CH2Cl2, EtOAc, acetone (10 mg/ml), and chloroform.
荧光
λex 335-362 nm, λem 505-512 nm; λex 369 nm, λem 471 nm in ethyl acetate(prior to hydrolysis of AM esters in cell)
应用
实验应用
A highly selective Ca2+ fluorescent chelator
背景
别名
钙离子荧光探针 Fura 2-AM
分子式
C44H47N3O24
CAS号
108964-32-5
沸点
975.91° C at 760 mmHg (Predicted)
MDL
MFCD00036976
密度
1.40 g/cm3
折射率
n20D 1.57 (Predicted)
制备和贮存
保存方式
Store at -20° C，2 years
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