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Fyn (Ab-530)
Fyn (Ab-530)
品牌： Usbiological
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对比
咨询纯度：
Purified by immunoaffinity chromatography.
反应种属：
Rabbit
产品介绍
产品介绍
产品信息
抗原来源
Human
宿主
Rabbit
免疫原
Synthesized non-phosphopeptide derived from human Fyn around the phosphorylation site of tyrosine 530 (P-Q-Y(p)-Q-P).
产品用途
Suitable for use in Western Blot. Other applications not tested.
简单描述
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.Kawakami T., Mol. Cell. Biol. 6:4195-4201(1986). Semba K., Proc. Natl. Acad. Sci. U.S.A. 83:5459-5463(1986). Rigley K., J. Immunol. 154:1136-1145(1995)
同种型
IgG
克隆类别
Polyclonal
纯度
Purified by immunoaffinity chromatography.
形式
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
应用
实验应用
WB
推荐用量
Western Blot: 1:500-1:3000 Optimal dilutions to be determined by the researcher.
反应种属
Rabbit
交叉反应性
Hu Mo
目标/特异性
Human Mouse
稀释度
Western Blot: 1:500-1:3000 Optimal dilutions to be determined by the researcher.
背景
别名
EC 2.7.10.2, FYN, kinase Fyn, P59-FYN, SLK
制备和贮存
保存方式
Storage_Temp:-20°C
Storage_and_Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
数据库链接
Entrez-Gene ID
2534
UniProt ID
P06241
SwissProt
P06241
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