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GAPDH (14C10) Rabbit mAb (HRP Conjugate)
品牌： CST
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对比
咨询分子量：
37
反应种属：
Human,Mouse,Rat,Monkey,Bovine,Pig,
产品介绍
产品信息
荧光素标记
抗原名称
GAPDH
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH. The antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
宿主
Rabbit
简单描述
Monoclonal Antibody for studying GAPDH. Cited in 380 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, GAPDH (14C10) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #3683) is ready to ship.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
分子量
37
内毒素水平
猪
研究领域
代谢,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,Bovine,Pig,
预测反应种属
Pig
目标/特异性
Specificity/Sensitivity
GAPDH (14C10) Rabbit mAb (HRP Conjugate) detects endogenous levels of total GAPDH protein.
Species Reactivity:
Human, Mouse, Rat, Monkey, Bovine, Pig
敏感性
Endogenous
背景
背景
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
1.Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2.Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3.Zheng, L. et al. (2003) Cell 114, 255-66.
4.Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5.Wang, Q. et al. (2005) FASEB J. 19, 869-71.
研究领域
代谢,神经科学,
翻译后修饰
Unmodified
制备和贮存
保存方式
Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
数据库链接
Entrez-Gene ID
2597
UniProt ID
P04406
参考图片
Western blot analysis of extracts from NIH/3T3, HeLa and C6 cells using GAPDH (14C10) Rabbit mAb (HRP Conjugate).
Wetern blot检测NIH/3T3, HeLa和 C6 细胞提取物，所用抗体为GAPDH (14C10) Rabbit mAb 兔单抗(HRP Conjugate)
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