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GAPDH (D16H11) XP ® Rabbit mAb

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GAPDH (D16H11) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

GAPDH

来源纯化

Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresponding to residues surrounding Lys260 of human GAPDH protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunohistochemistry (Paraffin)	1:400 - 1:1600
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400

同种型

Rabbit IgG

分子量

内毒素水平

猪

研究领域

癌症,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Monkey,

预测反应种属

Pig

目标/特异性

Specificity/Sensitivity

GAPDH (D16H11) XP Rabbit mAb detects endogenous levels of total GAPDH protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5). 1.Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95. 2.Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38. 3.Zheng, L. et al. (2003) Cell 114, 255-66. 4.Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9. 5.Wang, Q. et al. (2005) FASEB J. 19, 869-71. 6.Kozako, T. et al. (2015) Sci Rep 5, 11345.

研究领域

癌症,代谢,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #92310.

数据库链接

Entrez-Gene ID

2597

UniProt ID

P04406

参考图片

Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb.

Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb.

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货号：

5174S

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