Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresponding to residues surrounding Lys260 of human GAPDH protein.
宿主
Rabbit
简单描述
Monoclonal Antibody for studying GAPDH. Cited in 6310 publications. Validated for WB, WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, GAPDH (D16H11) XP® Rabbit Monoclonal Antibody (CST #5174) is ready to ship.
商品描述
Product Usage Information
Application
Dilution
Western Blotting
1:1000
Simple Western™
1:10 - 1:50
Immunohistochemistry (Paraffin)
1:800
Immunofluorescence (Immunocytochemistry)
1:200
分子量
37
内毒素水平
猪
研究领域
癌症,代谢,神经科学
应用
反应种属
Human,Mouse,Rat,Monkey,
预测反应种属
Pig
目标/特异性
Specificity/Sensitivity
GAPDH (D16H11) XP® Rabbit mAb detects endogenous levels of total GAPDH protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
1.Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2.Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3.Zheng, L. et al. (2003) Cell 114, 255-66.
4.Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5.Wang, Q. et al. (2005) FASEB J. 19, 869-71.
6.Kozako, T. et al. (2015) Sci Rep 5, 11345.
研究领域
癌症,代谢,神经科学
翻译后修饰
Unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #92310.
数据库链接
Entrez-Gene ID
2597
UniProt ID
P04406
参考图片
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb.
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
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