Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit mAb

Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7). 1.Kayagaki, N. et al. (2015) Nature 526, 666-71. 2.Shi, J. et al. (2015) Nature 526, 660-5. 3.Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20. 4.Aglietti, R.A. et al. (2016) Proc Natl Acad Sci U S A 113, 7858-63. 5.Ding, J. et al. (2016) Nature 535, 111-6. 6.Liu, X. et al. (2016) Nature 535, 153-8. 7.Sborgi, L. et al. (2016) EMBO J 35, 1766-78.