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咨询ELISA Capture (Matched Antibody Pair)(2-8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
Arg109-Ile211
Accession # P39905
Human GDNF Antibody Summary
Arg109-Ile211
Accession # P39905
Applications
Human GDNF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ELISA Capture (Matched Antibody Pair)(2-8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
Background: GDNF
Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons.
Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
参考图片
Proliferation Induced by GDNF and Neutralization by Human GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH‑SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by the Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDNF Monoclonal Antibody (Catalog # MAB212). The ND50 is typically 0.4‑2 μg/mL.