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General Vitamin B9 (VB9) ELISA Kit
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产品介绍
产品介绍
产品信息
商品描述
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of VB9 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to VB9 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled VB9 and unlabeled VB9 (Standards or samples) with the pre-coated antibody specific to VB9. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of VB9 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of VB9 in the sample.
应用
反应种属
General
检测范围
3.90-250ng/mL
检测类型
Competitive inhibition
样本类型以及上样量
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
敏感性
1.23ng/mL
背景
别名
VB9; Folic Acid; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate
制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
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