GFP-CERTIFIED® Apoptosis/Necrosis detection kit

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Application Note:Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential Neurotoxicants Cited samples:For an overview on cited samples please click here.

Multiplex assay that distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other fluorescent probes (blue or cyan) Specifically designed for use with GFP-expressing cell lines and cells expressing blue or cyan fluorescent proteins (BFPs, CFPs) Readily distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells Optimized for both fluorescence microscopy and flow cytometry applications Suitable for death pathway analysis and drug/toxin studies Suitable for use with live or post-fixed cells  Enzo Life Sciences GFP CERTIFIED® Apoptosis/Necrosis Detection Kit includes all the necessary reagents for determination of early and late stages of apoptosis as well as necrosis.  An Annexin V-EnzoGold (enhanced Cyanine-3) conjugate enables the detection of externalized phosphatidylserine (PS) (a marker of early apoptosis) distinct from fluorescein or GFP signals. The Necrosis Detection Reagent (Red), similarly to the red-emitting dye 7-AAD, diffuses into dead or damaged cells and binds to the DNA. Both Apoptosis Detection Reagent and Necrosis Detection Reagent are excluded from live cells with intact membranes. This kit also includes an Apoptosis Inducer (Staurosporine) for use as a positive control.

Flow Cytometry, Fluorescence microscopy, Fluorescent detection

Phosphatidylserine (PS)/DNA stain

Store the Annexin V-EnzoGold and Binding Buffer at 4°C. Store all other reagents at -20°C. Reconstituted inducer (staurosporine) should be stored at -20°C. Refer to manual.