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Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb

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Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro190 of human glutamate dehydrogenase 1 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:800 - 1:3200
Immunofluorescence (Immunocytochemistry)	1:600 - 1:1200

同种型

Rabbit IgG

分子量

应用

目标/特异性

Specificity/Sensitivity

Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb recognizes endogenous levels of total glutamate dehydrogenase 1 and 2 proteins. Species cross-reactivity for IHC-P and IF-IC is in human only.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Glutamate dehydrogenase is a mitochondrial enzyme that catalyzes the oxidative deamination of glutamate to α-ketoglutarate through association with the cofactor nicotinamide adenine dinucleotide phosphate (1). Glutamate dehydrogenase is highly expressed in various tissues such as the liver, brain, kidney, heart, pancreas, ovaries, and testis. Two isoforms produced by two distinct genes are found in mammalian tissues. The GLUD1 gene is ubiquitously expressed (2), while the GLUD2 gene is specifically expressed in testicular tissues and astrocytes (3,4). Glutamate dehydrogenase links glutamate to the Krebs cycle, thereby playing a critical role in the regulation of energy homeostasis. Research studies have shown that changes in glutamate dehydrogenase activity in pancreatic β-cells can cause a hyperinsulinism syndrome (5). 1.Blumenthal, K.M. et al. (1975) J Biol Chem 250, 3644-54. 2.Michaelidis, T.M. et al. (1993) Genomics 16, 150-60. 3.Shashidharan, P. et al. (1997) J Neurochem 68, 1804-11. 4.Zaganas, I. et al. (2012) Neurochem Int 61, 455-62. 5.Karaca, M. et al. (2011) Neurochem Int 59, 510-7.

研究领域

癌症,代谢,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #80063.

数据库链接

Entrez-Gene ID

2746

UniProt ID

P00367

参考图片

Confocal immunofluorescent analysis of A172 cells using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human glutamate dehydrogenase 1 (hGluD1; +) or glutamate dehydrogenase 2 (hGluD2; +), using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western blot analysis of extracts from various cell lines and tissues using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb.

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货号：

12793T

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20μl

100ul

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