Goat anti-Mouse IgG1 Heavy Chain Antibody Affinity Purified

Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity to mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3 and IgE is less than 1%. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.

Mouse IgG1 Heavy Chain

Mouse

Goat

IgG1 is the most dominant subclass of IgG and is known to be able to cross the placenta, bind strongly to Fc receptors, and activate the complement pathway efficiently.

IgG

Polyclonal

Antigen Affinity Purified

1 mg/ml

Liquid

ELISA, Enzyme-Linked Immunosorbent Assay (ELISA),Enzyme-Linked Immunosorbent Assay (ELISA),ICC, Immunocytochemistry (ICC),Immunocytochemistry (ICC),IHC, Immunohistochemistry (IHC),Immunohistochemistry (IHC),WB Western Blot (WB),Western Blot (WB)

Mouse