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ICC
1:500IF
1:500
N/A
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
参考图片
ICC shows positive staining in HeLa cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody (S0B0043) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
IF shows positive staining in paraffin-embedded human tonsil. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) (S0B4005) was used at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostate. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) (S0B4005) was used at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.