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DAPI is supplied as a lyophilized powder in 1 mg units. It can be used to examine cellular DNA in fluorescent microscopy and cytometry applications.
| Molecular Formula | C16H15N5 2(HCl) |
Product Usage Information
Directions for Use: DAPI is supplied as a lyophilized powder. For a 20 mg/ml stock, reconstitute the 1 mg in 50 µl deionized water or dimethylformamide. Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:Immunofluorescence: Counterstain with DAPI as the final step in your staining procedure. Rinse samples twice in PBS for five min each. Dilute DAPI stock solution to a concentration between 1 - 0.1 µg/ml in PBS and incubate for 5 min at room temperature in the dark. Rinse samples once in PBS and then prepare for imaging. Examine immediately using appropriate excitation wavelength.Alternatively, dilute DAPI stock solution to a concentration between 1 - 0.1 µg/ml in mounting media, apply to cells, and prepare for imaging. Examine immediately using appropriate excitation wavelength.Flow Cytometry: Rinse samples once in Incubation Buffer. Dilute DAPI stock solution to a concentration between 1.60-0.400 µg/ml in PBS and incubate for 15 min at room temperature in the dark before analyzing cells on flow cytometer.
Store lyophilized or in solution at 4°C, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 2-3 weeks to prevent loss of potency. For long-term storage, aliquot and store at -20°C to avoid multiple freeze/thaw cycles.
参考图片
Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 647 Conjugate) #12075 and DAPI (DNA content).
Immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green), β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red) and DAPI (blue).
Immunofluorescent analysis of HUVE cells using VE-Cadherin (D87F2) XP® Rabbit mAb #2500 (green) and DAPI (blue). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).