Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c protein.
宿主
Rabbit
简单描述
Monoclonal Antibody for studying cytochrome C. Cited in 337 publications. Validated for Western Blotting, Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Cytochrome c (D18C7) Rabbit Monoclonal Antibody (CST #11940) is ready to ship.
商品描述
Product Usage Information
Application
Dilution
Western Blotting
1:1000
Immunohistochemistry (Paraffin)
1:50 - 1:200
分子量
14
内毒素水平
牛 , 猪
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
预测反应种属
Bovine,Pig,
目标/特异性
Specificity/Sensitivity
Cytochrome c (D18C7) Rabbit mAb recognizes endogenous levels of total cytochrome c protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).
1.Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
2.Li, P. et al. (1997) Cell 91, 479-489.
3.Liu, X. et al. (1996) Cell 86, 147-157.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
Unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
54205
UniProt ID
P99999
参考图片
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Cytochrome c (D18C7) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
免疫组织化学染色分析石蜡包埋人乳腺癌组织。在对照多肽(左图)或者抗原特异性肽(右图)存在下,所用抗体为Cytochrome c (D18C7) Rabbit mAb 兔单抗。
Western blot analysis of extracts from various cell lines using Cytochrome c (D18C7) Rabbit mAb.
Western blot 检测多种细胞系提取物，使用抗体为 Cytochrome c (D18C7) Rabbit mAb 兔单抗。
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +). Cells were fractionated into whole cell lysate (WCL), cytoplasm (Cyto), membrane (Mem), and cytoskeletal/nucleus (Nuc). Membrane fraction includes mitochondria.
Western blot 检测HeLa细胞提取物，未处理 (-)或Staurosporine #9953 (1 μM, 3 hr; +)处理。细胞分离成整个细胞裂解液（WCL），细胞质（Cyto），膜（Mem）和细胞骨架/核（Nuc）。膜部分包括线粒体。
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using Cytochrome c (D18C7) Rabbit mAb.
免疫组织化学染色分析石蜡包埋小鼠骨骼肌切片，所用抗体为Cytochrome c (D18C7) Rabbit mAb 兔单抗。
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