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产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
抗原名称
GAPDH
来源纯化
Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresponding to residues surrounding Lys260 of human GAPDH protein.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
同种型
Rabbit IgG
分子量
37
内毒素水平
猪
研究领域
癌症,代谢,神经科学
应用
反应种属
Human,Mouse,Rat,Monkey,
预测反应种属
Pig
目标/特异性
Specificity/Sensitivity
GAPDH (D16H11) XP Rabbit mAb detects endogenous levels of total GAPDH protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
1.Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2.Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3.Zheng, L. et al. (2003) Cell 114, 255-66.
4.Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5.Wang, Q. et al. (2005) FASEB J. 19, 869-71.
6.Kozako, T. et al. (2015) Sci Rep 5, 11345.
研究领域
癌症,代谢,神经科学
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #92310.
数据库链接
Entrez-Gene ID
2597
UniProt ID
P04406
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