Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. 1.Montminy, M. (1997) Annu Rev Biochem 66, 807-22. 2.Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 3.Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8. 4.Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53. 5.Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4. 6.Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.