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Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb

品牌： CST

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产品介绍

产品信息

抗原名称

CoA Carboxylase

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying acetyl-CoA carboxylase 1 (Ser80) phosphate/acetyl-CoA carboxylase 2 (Ser222) phosphate. Cited in 338 publications. Validated for WB, IP, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit Monoclonal Antibody (CST #11818) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:200 - 1:800
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400

分子量

280

应用

反应种属

Human,Mouse,Rat,

目标/特异性

Specificity/Sensitivity

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.

Species Reactivity:

Human, Mouse, Rat

敏感性

Endogenous

背景

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5). 1.Castle, J.C. et al. (2009) PLoS One 4, e4369. 2.Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86. 3.Ha, J. et al. (1994) J Biol Chem 269, 22162-8. 4.Abu-Elheiga, L. et al. (2001) Science 291, 2613-6. 5.Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #42801.

数据库链接

Entrez-Gene ID

31,32

UniProt ID

Q13085,O00763

参考图片

Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

免疫组织化学分析石蜡包埋的NCI-H2228细胞，未经处理（左）或苯乙双胍处理（右），使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

免疫组织化学分析石蜡包埋的人肺癌细胞，未经处理（左）或λ磷酸酶处理（右），使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗。

Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment.

Western blot方法检测SH-SY5Y细胞提取物：未处理或使用Oligomycin #9996 处理(0.5 μM, 30 min)，使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗（上图）或 Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗 #3676（下图）。使用λ 磷酸酶验证抗体的磷酸化特异性。

Confocal immunofluorescent analysis of 293 cells (all nutrient-starved with Krebs-Ringer bicarbonate buffer for 4 hr), starved only (top left), serum-treated (10%, 30 min; top right), H2O2-treated (10 mM, 10 min; bottom left), or λ phosphatase-treated (2 hr; bottom right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析293细胞（所有饥饿处理为Krebs-Ringer碳酸氢盐缓冲液处理4hr），仅饥饿（左上），血清处理（10%, 30min；右上），过氧化氢处理（10 mM, 10min；左下），或λ磷酸酶处理（2hr，右下），用Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗（绿色）（Ser79）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。

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货号：

11818T

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