Phospho-RPA32/RPA2 (Ser33) (E9N1T) Rabbit mAb

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30, and 14 kDa subunits, collectively known as RPA. RPA is a single-stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch, and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Phosphorylation of RPA32 occurs at serines 4, 8, and 33 (11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA, and Pol α, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12). 1.Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222-31. 2.Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834-8. 3.Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211-8. 4.Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523-7. 5.Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433-9. 6.Kastan, M.B. and Bartek, J. (2004) Nature 432, 316-23. 7.Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39-85. 8.Guo, S. et al. (2006) J Biol Chem 281, 21607-16. 9.Wu, X. et al. (2005) Oncogene 24, 4728-35. 10.Binz, S.K. et al. DNA Repair (Amst) 3, 1015-24. 11.Nuss, J.E. et al. (2005) Biochemistry 44, 8428-37. 12.Yuzhakov, A. et al. (1999) EMBO J. 18, 6189-99.