The GB11 antibody specifically reacts with human granzyme B, a serine protease of approximately 32 kDa.
Granzyme B is stored in the granules of cytotoxic T lymphocytes and NK cells along with the pore-forming protein perforin. In the classic model of target cell lysis, perforins create holes in the target cell membrane allowing entrance of granzymes. Granzyme B has been shown to act on specific substrates including caspase-3, -7, -9, and -10 which in turn give rise to enzymes that mediate apoptosis. Granzyme B may also be involved in the hydrolysis of extracellular matrix components.
Detectable levels of granzyme B have been detected in sera from healthy volunteers. The immunogen used to generate the GB11 hybridoma was human granzyme B isolated from an NK cell line.
商品描述
GB11
The GB11 antibody specifically reacts with human granzyme B, a serine protease of approximately 32 kDa.
Granzyme B is stored in the granules of cytotoxic T lymphocytes and NK cells along with the pore-forming protein perforin. In the classic model of target cell lysis, perforins create holes in the target cell membrane allowing entrance of granzymes. Granzyme B has been shown to act on specific substrates including caspase-3, -7, -9, and -10 which in turn give rise to enzymes that mediate apoptosis. Granzyme B may also be involved in the hydrolysis of extracellular matrix components.
Detectable levels of granzyme B have been detected in sera from healthy volunteers. The immunogen used to generate the GB11 hybridoma was human granzyme B isolated from an NK cell line.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 GB11 (RUO)
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(9)
1. Hamann D, Baars PA, Rep MH. Phenotypic and functional separation of memory and effector human CD8+ T cells. J Exp Med. 1997; 186(9):1407-1418. (Clone-specific: Flow cytometry).
2. Poe M, Blake JT, Boulton DA. Human cytotoxic lymphocyte granzyme B. Its purification from granules and the characterization of substrate and inhibitor specificity. J Biol Chem. 1991; 266(1):98-103. (Biology).
3. Ronday HK, van der Laan WH, Tak PP et al. Human granzyme B mediates cartilage proteoglycan degradation and is expressed at the invasive front of the synovium in rheumatoid arthritis. Rheumatology (Oxford). 2001; 40:55-61. (Biology).
4. Smyth MJ, Kelly JM, Sutton VR et al. Unlocking the secrets of cytotoxic granule proteins. J Leukoc Biol. 2001; 70:18-29. (Biology).
5. Spaeny-Dekking EH, Hanna WL, Wolbink AM et al. Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998; 160:3610. (Biology).
6. Trapani JA, Klein JL, White PC, and Dupont B. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc Natl Acad Sci U S A. 1988; 5:6924-6928. (Biology).
7. Trapani JA, Smyth MJ, Apostolidis VA, Dawson M, and Browne KA. Granule serine proteases are normal nuclear constituents of natural killer cells. J Biol Chem. 1994; 269:18359-18365. (Biology).
8. Wever PC, Van Der Vliet HJ, Spaeny LH . The CD8+ granzyme B+ T-cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells. Immunology. 1998; 93(3):383-389. (Clone-specific: Flow cytometry).
9. ten Berge IJ, Wever PC, Rentenaar RJ. Selective expansion of a peripheral blood CD8+ memory T cell subset expressing both granzyme B and L-selectin during primary viral infection in renal allograft recipients. Transplant Proc. 1998; 30(8):3975-3977. (Biology).
数据库链接
Entrez-Gene ID
3002, 14939
参考图片
Expression of granzyme B by CD8 positive and CD8 negative peripheral blood mononuclear cells. Whole human blood was lysed with PharmLyse™ Lysing buffer (Cat. No. 555899) prior to staining with GB11. The lysed human blood was subsequently fixed, permeablilized and stained with APC- conjugated mouse anti-human CD8 (APC- RPA-T8, Cat. No. 555369) and either mouse anti-human granzyme B antibody (FITC- GB11, Cat. No. 558132), (filled histograms) or immunoglobulin isotype control (FITC- MOPC-21, Cat. No. 555909), (empty histograms) by using Pharmingen's staining protocol. To demonstrate specificity of staining, the binding of FITC-GB11 was blocked by preincubation of the fixed/permeabilized cells with an excess of unlabelled GB11 antibody (5 µg, data not shown) prior to staining. The histograms in the figure were derived from CD8-positive (Left panel) or CD8 -negative (Right panel) gated events.
Expression of granzyme B by CD8 positive and CD8 negative peripheral blood mononuclear cells. Whole human blood was lysed with PharmLyse™ Lysing buffer (Cat. No. 555899) prior to staining with GB11. The lysed human blood was subsequently fixed, permeablilized and stained with APC- conjugated mouse anti-human CD8 (APC- RPA-T8, Cat. No. 555369) and either mouse anti-human granzyme B antibody (FITC- GB11, Cat. No. 558132), (filled histograms) or immunoglobulin isotype control (FITC- MOPC-21, Cat. No. 555909), (empty histograms) by using Pharmingen's staining protocol. To demonstrate specificity of staining, the binding of FITC-GB11 was blocked by preincubation of the fixed/permeabilized cells with an excess of unlabelled GB11 antibody (5 µg, data not shown) prior to staining. The histograms in the figure were derived from CD8-positive (Left panel) or CD8 -negative (Right panel) gated events.
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