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产品介绍
产品信息
抗原名称
GST-Tag
来源纯化
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
宿主
Rabbit
简单描述
Monoclonal Antibody for studying GST. Cited in 152 publications. Validated for Western Blotting, Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GST-Tag (91G1) Rabbit Monoclonal Antibody (CST #2625) is ready to ship.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
应用
反应种属
All
目标/特异性
Specificity/Sensitivity
GST-Tag (91G1) Rabbit mAb detects transfected GST fusion proteins.
Species Reactivity:
All Species Expected
敏感性
Transfected Only
背景
背景
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
1.Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
2.Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
3.Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
翻译后修饰
Unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing GST-Bad (+), using GST (91G1) Rabbit mAb (left) and Bad Antibody #9292 (right).Western blot 分析未转染（-）和转染过表达GST-Bad蛋白（+）的细胞提取物。所用抗体为GST (91G1) Rabbit mAb (左图) and Bad Antibody #9292 (右图).
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