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GST-Tag (91G1) Rabbit mAb

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GST-Tag (91G1) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

GST-Tag

来源纯化

Monoclonal antibody is produced by immunizing animals with a GST fusion protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:200

同种型

Rabbit IgG

应用

反应种属

All

目标/特异性

Specificity/Sensitivity

GST-Tag (91G1) Rabbit mAb detects transfected GST fusion proteins.

Species Reactivity:

All Species Expected

敏感性

Transfected Only

背景

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3). 1.Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7. 2.Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6. 3.Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

参考图片

Simple Western™ analysis of lysates (0.1 mg/mL) from 293T cells, transfected with a construct expressing GST tagged BetaTrop1/trc using GST-Tag (91G1) Rabbit mAb #2625. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Western blot analysis of extracts from COS-7 cells untransfected (-) and transfected cells overexpressing GST-Bad (+), using GST-Tag (91G1) Rabbit mAb (left) and Bad Antibody #9292 (right).

Western blot analysis of recombinant human GST-MST3 at various concentrations, using GST-Tag (91G1) Rabbit mAb.

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货号：

2625S

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20ul

100ul

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