全部商品分类
![BD OptiBuild™ RY586 Mouse Anti-Mouse H-2K[b]/H-2D[b]](https://univ-pmc.oss-cn-shanghai.aliyuncs.com/uprod.collection.product/spider/bd/d30bfa72-f305-3731-8d20-8b461fed6907.jpg)
品牌： BD Pharmingen
下载产品说明书 下载SDS
用小程序，查商品更便捷
收藏
对比
咨询反应种属：
Mouse (Tested in Development)
来源宿主：
Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
H-2Kb, H-2Db
宿主
Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ
免疫原
C3H.SW mouse splenocytes
简单描述
The 28-8-6 monoclonal antibody specifically recognizes the H-2K[b] and H-2D[b] MHC class I alloantigens. The reaction with H-2K[b] has been reported to be stronger than that with H-2D[b]. Its recognition of H-2D[b] is dependent upon the presence of β2 microglobulin. Cross-reactivity with an epitope on the N-terminal domains, α1 and α2, of the H-2D[d] MHC class I alloantigen has also been described. Reactivity with other haplotypes (
e.g., f, k, p, q, r, s
) has not been observed.
商品描述
28-8-6
The 28-8-6 monoclonal antibody specifically recognizes the H-2K[b] and H-2D[b] MHC class I alloantigens. The reaction with H-2K[b] has been reported to be stronger than that with H-2D[b]. Its recognition of H-2D[b] is dependent upon the presence of β2 microglobulin. Cross-reactivity with an epitope on the N-terminal domains, α1 and α2, of the H-2D[d] MHC class I alloantigen has also been described. Reactivity with other haplotypes (
e.g., f, k, p, q, r, s
) has not been observed.
同种型
Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ
克隆号
克隆 28-8-6 (RUO)
浓度
0.2 mg/ml
产品详情
RY586
The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
RY586
Yellow-Green 561 nm
564 nm
586 nm
应用
实验应用
Flow cytometry (Qualified)
反应种属
Mouse (Tested in Development)
目标/特异性
H-2Kb, H-2Db
背景
别名
H-2Kb; H-2Db; Histocompatibility-2Kb; Histocompatibility-2Db
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Allen H, Fraser J, Flyer D, Calvin S, Flavell R. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A. 1986; 83(19):7447-7451. (Biology).
2. Evans GA, Margulies DH, Shykind B, Seidman JG, Ozato K. Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens. Nature. 1982; 300(5894):755-757. (Biology).
3. Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981; 126(1):317-321. (Immunogen).
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。