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品牌： Absin
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对比
咨询分子量：
Detects a band of approximately 20kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
反应种属：
Human, Mouse, Rabbit
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
The antibody was purified by immunogen affinity chromatography.
宿主
Rabbit
免疫原
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S139 of human Histone H2A.X protein. The exact sequence is proprietary.
商品描述
Rabbit polyclonal antibody to Histone H2A.X (Phospho-S139).
分子量
Detects a band of approximately 20kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
克隆类别
Polyclonal Antibody
应用
实验应用
WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:50-1:200, ICC: 1:50-1:200, FCM: 1:100-1:200
反应种属
Human, Mouse, Rabbit
目标/特异性
Recognizes endogenous levels of Histone H2A.X protein only when phosphorylated at S139.
背景
别名
Histone H2A.X抗体
翻译后修饰
Phosphorylated
制备和贮存
存储溶液
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
保存方式
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
参考图片
Western blot analysis of Histone H2A.X (pS139) expression in HEK293T (A), NIH3T3 (B) whole cell lysates.
Immunohistochemical analysis of Histone H2A.X (pS139) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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