CST_HA-Tag(C29F4) Rabbit mAb_优宁维(univ)在线
现货 HA-Tag(C29F4) Rabbit mAb
优宁维&CST全国五大现货仓 查看详情>
货号: 3724S 品牌: CST 库存: 公司现货
规格: 100ul
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价格: ¥3780.00

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产品信息
来源纯化
通过使用含有流感血凝素表位 (YPYDVPDYA) 的合成肽对动物进行免疫接种来产生单克隆抗体。
宿主
Rabbit
应用
敏感性
转染性
背景
背景
在使用免疫印迹法、免疫沉淀法和免疫染色技术对蛋白进行标记和检测时,表位标签十分有用。由于分子量小,它们不太可能对标记蛋白的生化特性造成影响。HA 标签衍生自流感血凝素蛋白的表位,该表位已经广泛用作表达载体中的通用表位标签 (1)。 Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.
抗原
HA

参考图片

Flow cytometric analysis of HeLa cells, untransfected (blue) or transfected with HA-FoxO4 (green), using HA-Tag (C29F4) Rabbit mAb #3724.流式细胞术分析HeLa细胞,蓝色为未转染组,绿色为HA-FoxO4 转染组,所用抗体为HA-Tag (C29F4) Rabbit mAb #3724。

Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (right) or HA-Tag transfected (left), using HA-Tag (C29F4) Rabbit mAb.免疫组化分析石蜡包埋的COS细胞,下图为未转染组,上图为HA-Tag转染组,所用抗体为HA-Tag (C29F4) Rabbit mAb。

Western blot analysis of extracts from HeLa cells, untransfected or transfected with either HA-FoxO4 or HA-Akt3, using HA-Tag (C29F4) Rabbit mAb.Weatern blot分析HeLa细胞提取物,未转染组和采用 HA-FoxO4 或 HA-Akt3转染组,所用抗体为HA-Tag (C29F4) Rabbit mAb。

Confocal immunofluorescent analysis of COS cells, transfected with an HA-tagged protein (left) or mock-transfected (right), using HA-Tag (C29F4) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).免疫荧光分析COS-7细胞提取物。左图为采用HA标签蛋白转染组,下图为mock转染组,所用抗体为HA-Tag (C29F4) Rabbit (绿色),肌动蛋白丝采用DY-554 phalloidin (红色)标记。蓝色伪彩为荧光DNA染料,信息为DRAQ5 ® #4084 。

293T cells were either untransfected (left panel) or transfected with an HA-tagged human CBP construct (right panel), then treated with Forskolin #3828 (30 µM) . Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 10 µl of HA-Tag (C29F4) Rabbit mAb, 10 µl of CBP (D9B6) Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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