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产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
抗原名称
HA-Tag
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).
宿主
Rabbit
商品描述
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:800 - 1:3200 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:1600 |
| Chromatin IP | 1:50 |
同种型
Rabbit IgG
应用
反应种属
All
目标/特异性
Specificity/Sensitivity
HA-Tag (C29F4) Rabbit mAb detects exogenously expressed proteins containing the HA epitope tag. The antibody may cross-react with a protein of unknown origin ~100kDa.
Species Reactivity:
All Species Expected
敏感性
Transfected Only
背景
背景
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1).
1.Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #86124.
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