R&D Systems_hb-NGF Biot Aff Pur (50 ug)_优宁维(univ)在线
hb-NGF Biot Aff Pur (50 ug)
货号: BAF256 品牌: R&D Systems 反应种属:
Human
Human
应用:
Western Blot(0.1 µg/mL)
ELISA Capture (Matched Antibody Pair)(2-8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
Western Blot(0.1 µg/mL)
ELISA Capture (Matched Antibody Pair)(2-8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
规格: 50ug
产品说明书
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价格: ¥5919.00

数量
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产品信息
耦联标记
Biotin
纯化方式
Antigen Affinity-purified
目标/特异性
Detects human beta -NGF in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant mouse beta ‑NGF is observed and less than 3% cross-reactivity with recombinant rat beta ‑NGF is observed.
商品描述
For ELISA the Antibody Pairs information:Capture antibody:MAB256;Detection antibody:BAF256; and protein: 256-GF-100
产品形式
Reconstitute at 0.2 mg/mL in sterile PBS.
应用
实验应用
Western Blot(0.1 µg/mL)
ELISA Capture (Matched Antibody Pair)(2-8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
反应种属
Human
背景
背景

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

别名
Beta nerve growth factor precursor; beta-nerve growth factor; Beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428
制备和贮存
溶解方法
Reconstitute at 0.2 mg/mL in sterile PBS.
文献
文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Type: Cell Culture Supernates
Application: Luminex Development
Nerve growth factor and receptor expression in rheumatoid arthritis and spondyloarthritis.
Authors: Barthel C, Yeremenko N, Jacobs R
Arthritis Res. Ther., 2009;11(3):R82.
Species: Human
Sample Type: Whole Cells
Application: Flow
Nerve growth factor induces increased airway inflammation via a neuropeptide-dependent mechanism in a transgenic animal model of allergic airway inflammation.
Authors: Quarcoo D, Schulte-Herbruggen O, Lommatzsch M, Schierhorn K, Hoyle GW, Renz H, Braun A
Clin. Exp. Allergy, 2004;34(7):1146-51.
Species: Human bNGF transgenic mice
Sample Type: BALF
Application: ELISA Development
Nerve growth factor overexpression and autocrine loop in breast cancer cells.
Authors: Dolle L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H
Oncogene, 2003;22(36):5592-601.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development

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