老带新
hb-NGF Biot Aff Pur (50 ug)
1/3

hb-NGF Biot Aff Pur (50 ug)

分享
品牌: R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序,查商品更便捷
收藏
对比对比
咨询咨询
来源宿主:
Goat IgG
实验应用:
Western Blot(0.1 µg/mL)
ELISA Capture (Matched Antibody Pair)(2-8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Antigen Affinity-purified
抗原名称
Beta nerve growth factor precursor, beta-nerve growth factor, betaNGF, beta-NGF, HSAN5, MGC161426, n
宿主
Goat IgG
免疫原
Mouse myeloma cell line NS0-derived recombinant human beta -NGF (R&D Systems, Catalog # 256-GF)
Ser122-Ala239
Accession # CAA36832

Scientific Data

Flow Cytometry Detection of Human beta-NGF by Flow CytometryView Larger

Detection of Human beta-NGF by Flow Cytometry NGF staining by flow cytometry. PBMC of (a) healthy controls (HC) and (b) synovial fluid mononuclear cells (SFMC) from spondyloarthritis (SpA), and (c) rheumatoid arthritis (RA) patients were first stained with surface markers (CD3 and CD14) and permeabilized in order to enable intracellular detection of nerve growth factor (NGF). The cells were analysed by flow cytometry after setting lymphocyte (left column) and monocyte (right column) gates according to forward scatter vs side scatter properties of the cells. Dot plots of one representative individual of each group are shown. Image collected and cropped by CiteAb from the following publication (https://arthritis-research.biomedcentral.com/articles/10.1186/ar2716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human beta-NGF by Flow CytometryView Larger

Detection of Human beta-NGF by Flow Cytometry NGF staining by flow cytometry. PBMC of (a) healthy controls (HC) and (b) synovial fluid mononuclear cells (SFMC) from spondyloarthritis (SpA), and (c) rheumatoid arthritis (RA) patients were first stained with surface markers (CD3 and CD14) and permeabilized in order to enable intracellular detection of nerve growth factor (NGF). The cells were analysed by flow cytometry after setting lymphocyte (left column) and monocyte (right column) gates according to forward scatter vs side scatter properties of the cells. Dot plots of one representative individual of each group are shown. Image collected and cropped by CiteAb from the following publication (https://arthritis-research.biomedcentral.com/articles/10.1186/ar2716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human beta-NGF by Flow CytometryView Larger

Detection of Human beta-NGF by Flow Cytometry NGF staining by flow cytometry. PBMC of (a) healthy controls (HC) and (b) synovial fluid mononuclear cells (SFMC) from spondyloarthritis (SpA), and (c) rheumatoid arthritis (RA) patients were first stained with surface markers (CD3 and CD14) and permeabilized in order to enable intracellular detection of nerve growth factor (NGF). The cells were analysed by flow cytometry after setting lymphocyte (left column) and monocyte (right column) gates according to forward scatter vs side scatter properties of the cells. Dot plots of one representative individual of each group are shown. Image collected and cropped by CiteAb from the following publication (https://arthritis-research.biomedcentral.com/articles/10.1186/ar2716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human beta -NGF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -NGF in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant mouse beta ‑NGF is observed and less than 3% cross-reactivity with recombinant rat beta ‑NGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta -NGF (R&D Systems, Catalog # 256-GF)
Ser122-Ala241
Accession # CAA36832
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human beta -NGF (Catalog # 256-GF)

Human beta -NGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human beta-NGF Antibody (Catalog # MAB256)

Capture Reagent: Human beta ‑NGF Antibody (Catalog # MAB2561)

Standard: Recombinant Human beta-NGF Protein (Catalog # 256-GF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Western Blot(0.1 µg/mL)
ELISA Capture (Matched Antibody Pair)(2-8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
背景
别名
Beta nerve growth factor precursor,beta-nerve growth factor,betaNGF,beta-NGF,HSAN5,MGC161426,nerve growth factor (beta polypeptide),nerve growth factor, beta subunit,NGFB,NGFBMGC161428,beta Nerve Growth Factor
背景

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428
制备和贮存
溶解方法
Reconstitute at 0.2 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
声明 :本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。
货号:
BAF256
一键复制
询价
50ug
选择数量
当前规格1件起购 
配送至
预计2-3周送达,快递: 免运费,若需干冰额外收费