The BD IMag™ Mouse Hematopoietic Progenitor Cell Enrichment Set - DM reacts with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, and erythrocytes. The BD IMag™ Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. This Set is designed for the immunomagnetic enrichment of hematopoietic progenitors from mouse bone marrow by depletion of cells committed to the T- and B-lymphocytic, myeloid (monocytic and granulocytic), and erythroid lineages. The Set contains sufficient reagents to label 10^9 bone marrow cells.
Biotin Mouse Lineage Depletion Cocktail is comprised of the following biotin-conjugated monoclonal antibodies:
Anti-mouse CD3e, clone 145-2C11
Anti-mouse CD11, clone M1/70
Anti-mouse CD45R/B220, clone RA3-6B2
Anti-mouse Ly-6G and Ly-6C (Gr-1), clone RB6-8C5
Anti-mouse TER-119/Erythroid Cells, clone TER-119
克隆号
(RUO)
BD化合物表
描述
EntrezGene ID
Biotin Mouse Lineage Depletion Cocktail
N/A
Streptavidin Particles Plus - DM
N/A
应用
实验应用
Cell separation (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD3e, CD45R, Ly-76, CD11, Ly-6G, Ly6C
文献
文献
研发参考(6)
1. Goodell MA, Rosenzweig M, Kim H, et al. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species. Nat Med. 1997; 3(12):1337-1345. (Biology).
2. Morrison SJ, Wandycz AM, Hemmati HD, Wright DE, Weissman IL. Identification of a lineage of multipotent hematopoietic progenitors. Development. 1997; 124(10):1929-1939. (Biology).
3. Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5.
4. Sato T, Laver JH, Ogawa M. Reversible expression of CD34 by murine hematopoietic stem cells. Blood. 1999; 94(8):2548-2554. (Biology).
5. Spangrude GJ, Heimfeld S, Weissman IL. Purification and characterization of mouse hematopoietic stem cells. Science. 1988; 241(4861):58-62. (Biology).
6. Spangrude GJ, Scollay R. A simplified method for enrichment of mouse hematopoietic stem cells. Exp Hematol. 1990; 18(8):920-926. (Biology).
参考图片
Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with the BD IMag™ Mouse Hematopoietic Progenitor Enrichment Set (Cat. No. 557451) and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the accompanying Protocol. To demonstrate the efficiency of the depletion, unmanipulated bone marrow cells and the final depleted fraction were stained with APC Rat Anti-Mouse CD117 (Cat. No. 553356) to detect hematopoietic progenitors, and with PE Rat Anti-Mouse TER-119/Erythroid Cells (Cat. No. 553673), CD45R/B220 (Cat. No. 553089/553090), and CD11b (Cat. No. 557397/553311) to detect lineage-commited cells. The percentage of positive cells is indicated in each panel; placement of each marker is based upon staining with the appropriate isotype control (data not shown). The final depleted fraction contains a greatly increased proportion of CD117+ cells and less than 5% of lineage-positive contaminants.
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