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Hematoxylin is a blue nuclear counterstain for use in immunohistochemical assays. It yields crisp staining detail with superior contrast when used in conjunction with SignalStain® DAB Substrate Kit #8059. It is also compatible with SignalStain® Mounting Medium #14177.
Product Usage Information
Hematoxylin is a blue nuclear counterstain for use after the completion of immunohistochemical assays. It yields crisp nuclear staining with superior contrast with SignalStain® DAB Substrate Kit #8059 and is also compatible with SignalStain® Mounting Medium #14177.A) Solutions and ReagentsSupplied Reagents1. Hematoxylin #14166Additional Reagents (Not Supplied)1. Acid Rinse Solution: To prepare 100ml, add 2ml of glacial acetic acid to 98 ml of dH20.2. Bluing Solution: To prepare 100ml, add 1.5 ml of 30% ammonium hydroxide (NH4OH) to 98.5ml of 70% ethanol3. SignalStain® Mounting Medium #14177B) Nuclear Counterstaining1. Rinse slides in tap water.2. Completely submerse slides in Hematoxylin #14166 and incubate for 1-5 minutes.3. Wash slides with tap water until rinse water is clear.4. Immerse slides 10 times in acid rinse solution.5. Rinse slides 10 times in tap water.6. Immerse slides for 1 minute in bluing solution7. Rinse slides 10 times in tap water.8. Mount sections with SignalStain® Mounting Medium #14177.
Supplied as a working solution. Store at room temperature. Product is stable for 12 months upon receipt when stored properly.
参考图片
Chemical structure of Hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Claudin-1 (D5H1D) XP® Rabbit mAb #13255 (brown) followed by a counterstain with Hematoxylin (blue).