Hepatitis C Virus Core, aa2-80 (HCV Core)

Affinity Purified Purified by affinity chromatography.

Recombinant HCV core antigen consisting of aa2-192 (22kD) with beta-galactosidase (114kD) fused at the N-terminus.

The hepatitis C virus particle consists of a core of genetic material (RNA), surrounded by an icosahedral protective shell of protein, and further encased in a lipid (fatty) envelope of cellular origin. Two viral envelope glycoproteins, E1 and E2, are embedded in the lipid envelope. At the 5' and 3' ends of the RNA are the UTR, that are not translated into proteins but are important to translation and replication of the viral RNA. The 5' UTR has a ribosome binding site[3] (IRES-Internal ribosome entry site) that starts the translation of a very long protein containing about 3,000 amino acids. The core domain of the hepatitis C virus (HCV) IRES contains a four-way helical junction that is integrated within a predicted pseudoknot. The conformation of this core domain constrains the open reading frame's orientation for positioning on the 40S ribosomal subunit. The large pre-protein is later cut by cellular and viral proteases into the 10 smaller proteins that allow viral replication within the host cell, or assemble into the mature viral particles. Structural proteins made by the hepatitis C virus include Core protein, E1 and E2; nonstructural proteins include NS2, NS3, NS4, NS4A, NS4B, NS5, NS5A, and NS5B.

8.V.020

Supplied as a liquid in 1X PBS, pH 7.2, 0.01% sodium azide., before the addition of glcyerol to 40%.

Monoclonal

ELISA (EL/EIA), Western Blot (WB)

Anti -Hepatitis C Virus Core, aa2-80 (HCV Core)