全部商品分类
用小程序，查商品更便捷
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the carboxy terminus of human ErbB3 protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:50 |
| IHC Leica Bond | 1:50 |
| Immunohistochemistry (Paraffin) | 1:125 - 1:500 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #60638.
参考图片
Flow cytometric analysis of MDA-MB-231 cells (blue, negative) and T-47D cells (green, positive) using HER3/ErbB3 (D22C5) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Western blot analysis of extracts from MCF7 (HER3+) and MDA-MB-231 (HER3-) cells using HER3/ErbB3 (D22C5) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Simple Western™ analysis of lysates (1.0 mg/mL) from MCF-7 cells using HER3/ErbB3 (D22C5) XP® Rabbit mAb #12708. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 44-660 kDa separation module.
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb performed on the Leica BOND Rx.
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb performed on the Leica BOND Rx.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb performed on the Leica BOND Rx.
Immunohistochemical analysis of paraffin-embedded non-small cell lung carcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded breast carcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded ovarian serous adenocarcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded 293T cell pellets transfected with human erbB family members HER3, EGFR, HER2 and HER4 (from left to right as indicated) using HER3/ErbB3 (D22C5) XP® Rabbit mAb (top panels). Transfections were confirmed using EGF Receptor (D38B1) XP® Rabbit mAb #4267 (lower left), HER2/ErbB2 (D8F12) XP® Rabbit mAb #4290 (lower middle) and HER4/ErbB4 (111B2) Rabbit mAb #4795 (lower right).
Immunohistochemical analysis of paraffin-embedded MCF7 (left) or MDA-MB-231 (right) cell pellets using HER3/ErbB3 (D22C5) XP® Rabbit mAb.
Confocal immunofluorescent analysis of MCF7 cells (HER3+, left) and MDA-MB-231 cells (HER-, right) using HER3/ErbB3 (D22C5) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).