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品牌: BD Pharmingen









反应种属:
Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog, Rabbit (Tested in Development)
Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog, Rabbit (Tested in Development)
来源宿主:
Mouse IgG2b, κ
Mouse IgG2b, κ
产品介绍
产品信息
耦联标记
FITC

抗原名称
HLA-DR

宿主
Mouse IgG2b, κ

免疫原
Human PBMC and SL6866 Cell Line

简单描述
The TU36 monoclonal antibody specifically binds to a monomorphic epitope on major histocompatibility complex (MHC) Class II HLA-DR antigens expressed on B cells, activated T cells, and antigen presenting cells. The antibody detects the MHC Class II αβ complex and not the isolated α or β chains. This antibody fixes complement.

商品描述
TU36
The TU36 monoclonal antibody specifically binds to a monomorphic epitope on major histocompatibility complex (MHC) Class II HLA-DR antigens expressed on B cells, activated T cells, and antigen presenting cells. The antibody detects the MHC Class II αβ complex and not the isolated α or β chains. This antibody fixes complement.

同种型
Mouse IgG2b, κ

克隆号
克隆 TU36 (also known as Tü36; TÜ36) (RUO)

产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)

推荐用量
20 µl

反应种属
Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog, Rabbit (Tested in Development)

目标/特异性
HLA-DR

制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Pawelec G, Ziegler A, Wernet P. Dissection of human allostimulatory determinants with cloned T cells: stimulation inhibition by monoclonal antibodies TU22, 34, 35, 36, 37, 39, 43, and 58 against distinct human MHC class II molecules. Hum Immunol. 1985; 12(3):165-176. (Biology).
2. Ziegler A, Heinig J, Muller C, et al. Analysis by sequential immunoprecipitations of the specificities of the monoclonal antibodies TU22,34,35,36,37,39,43,58 and YD1/63.HLK directed against human HLA class II antigens. Immunobiology. 1986; 171(1-2):77-92. (Biology).
3. Ziegler A, Uchańska-Ziegler B, Zeuthen J, Wernet P. HLA antigen expression at the single cell level on a K562 X B cell hybrid: an analysis with monoclonal antibodies using bacterial binding assays.. Somatic Cell Genet. 1982; 8(6):775-89. (Immunogen).

参考图片
Flow cytometric analysis of HLA-DR expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse Anti-Human HLA-DR (Cat. No. 555560; solid line histogram) or FITC Mouse IgG2b κ Isotype Control (Cat. No. 555742; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
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