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BD Horizon™ BV510 Mouse Anti-Human HLA-DR

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BD Horizon™ BV510 Mouse Anti-Human HLA-DR

品牌： BD Pharmingen

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反应种属：

Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development), Dog (Reported)

来源宿主：

Mouse IgG2a, κ

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产品介绍

产品信息

耦联标记

BV510

抗原名称

HLA-DR

宿主

Mouse IgG2a, κ

简单描述

The G46-6 monoclonal antibody specifically binds to HLA-DR, a major histocompatibility complex (MHC) class II antigen. HLA-DR antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6. HLA-DR is a transmembrane heterodimeric glycoprotein composed of an α chain (36 kDa) and a β subunit (27 kDa) expressed primarily on antigen presenting cells: B cells, dendritic cells, monocytes, macrophages, and thymic epithelial cells. HLA-DR is also expressed on activated T cells. This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4-positive T cells. The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

商品描述

G46-6 The G46-6 monoclonal antibody specifically binds to HLA-DR, a major histocompatibility complex (MHC) class II antigen. HLA-DR antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6. HLA-DR is a transmembrane heterodimeric glycoprotein composed of an α chain (36 kDa) and a β subunit (27 kDa) expressed primarily on antigen presenting cells: B cells, dendritic cells, monocytes, macrophages, and thymic epithelial cells. HLA-DR is also expressed on activated T cells. This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4-positive T cells. The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

同种型

Mouse IgG2a, κ

克隆号

克隆 G46-6 (also known as L243) (RUO)

产品详情

BV510

The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:BV510

激发源:Violet 405 nm

最多激发源:327 nm, 405 nm

最多发射源:512 nm

应用

实验应用

Flow cytometry (Routinely Tested)

推荐用量

5 µl

反应种属

Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development), Dog (Reported)

目标/特异性

HLA-DR

背景

别名

MHC class II antigen; HLA class II histocompatibility antigen

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(5) 1. Dieckmann D, Plottner H, Berchtold S, Berger T, Schuler G. Ex vivo isolation and characterization of CD4(+)CD25(+) T cells with regulatory properties from human blood. J Exp Med. 2001; 193(11):1303-1310. (Clone-specific: Flow cytometry). 2. Ibisch C, Pradal G, Bach JM, Lieubeau B. Functional canine dendritic cells can be generated in vitro from peripheral blood mononuclear cells and contain a cytoplasmic ultrastructural marker.. J Immunol Methods. 2005; 298(1-2):175-82. (Clone-specific). 3. Kitani A, Chua K, Nakamura K, Strober W. Activated self-MHC-reactive T cells have the cytokine phenotype of Th3/T regulatory cell 1 T cells. J Immunol. 2000; 165(2):691-702. (Clone-specific: Flow cytometry). 4. Moran TP, Collier M, McKinnon KP, Davis NL, Johnston RE, Serody JS. A novel viral system for generating antigen-specific T cells. J Immunol. 2008; 175(5):3431-3438. (Clone-specific: Flow cytometry). 5. Sorg RV, Kogler G, Wernet P. Identification of cord blood dendritic cells as an immature CD11c- population. Blood. 1999; 93(7):2302-2307. (Clone-specific: Flow cytometry).

参考图片

Flow cytometric analysis of HLA-DR expression on human peripheral blood lymphocytes and monocytes. Human whole blood was stained with the BD Horizon™ BV510 Mouse Anti-Human HLA-DR antibody (Cat. No. 563083; solid line histogram) or with a BD Horizon™ BV510 Mouse IgG2a, κ Isotype Control (Cat. No. 563027; dashed line histogram). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel) or monocytes (Right Panel). Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Flow cytometric analysis of HLA-DR expression on human peripheral blood lymphocytes and monocytes. Human whole blood was stained with the BD Horizon™ BV510 Mouse Anti-Human HLA-DR antibody (Cat. No. 563083; solid line histogram) or with a BD Horizon™ BV510 Mouse IgG2a, κ Isotype Control (Cat. No. 563027; dashed line histogram). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel) or monocytes (Right Panel). Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

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货号：

563083

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