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品牌: Rockland
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This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(ab’)2. No reaction was observed against anti-Horse IgG F(c) or anti-Papain.
来源宿主:
Horse
产品介绍
产品介绍
产品信息
宿主
Horse

简单描述
Horse IgG Fab Fragment - 008-0105

商品描述
Horse IgG Fab Fragment - 008-0105

同种型
IgG Fab

纯度
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(ab’)2. No reaction was observed against anti-Horse IgG F(c) or anti-Papain.

浓度
2.0 mg/mL by UV absorbance at 280 nm

形式
Liquid (sterile filtered)

防腐剂
0.01% (w/v) Sodium Azide

缓冲体系
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

产品类型
Native Protein

应用
实验应用
SDS-PAGE;Horse IgG Fab Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

目标/特异性
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(ab’)2. No reaction was observed against anti-Horse IgG F(c) or anti-Papain.

背景
别名
Horse IgG Fab Fragment

背景
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

保存方式
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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