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BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD28
品牌： BD Pharmingen
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对比
咨询反应种属：
Human (QC Testing), Rhesus,Cynomolgus,Baboon (Tested in Development)
来源宿主：
Mouse C3H x BALB/c IgG1, κ
产品介绍
产品介绍
产品信息
抗原名称
CD28
宿主
Mouse C3H x BALB/c IgG1, κ
免疫原
Human CD28 Transfected Cell Line
简单描述
The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells.
商品描述
CD28.2
The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells.
同种型
Mouse C3H x BALB/c IgG1, κ
克隆号
克隆 CD28.2 (RUO)
浓度
1.0 mg/ml
产品详情
NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
应用
实验应用
Flow cytometry (Routinely Tested), (Co)-stimulation (Tested During Development)
反应种属
Human (QC Testing), Rhesus,Cynomolgus,Baboon (Tested in Development)
目标/特异性
CD28
背景
别名
CD28 antigen; T44; Tp44; TP44
制备和贮存
存储溶液
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2 µm sterile filtered. Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
保存方式
No azide/low endotoxin: Aqueous buffered solution containing no preservative, sterile filtered(0.2µm pore size membrane). Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
数据库链接
Entrez-Gene ID
940, 705313, 100126740, 102146670
参考图片
Profile of peripheral blood lymphocytes analyzed by flow cytometry. Second step staining using FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988).
Flow cytometric analysis of CD28 expression by Human peripheral blood lymphocytes. Human whole blood was stained with Purified NA/LE Mouse Anti-Human CD28 antibody (Cat. No. 555725/567116/567117; solid line histogram). The cells were washed and then counterstained with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A histogram showing the expression of CD28 (or Ig Isotype control staining shown as dashed line histogram) was generated from gated events with the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD Flow Cytometer System.
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