Human Alpha Galactosyl ELISA Kit

Intended Uses: This alpha-GLA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human alpha-GLA. This ELISA kit for research use only, not for therapeutic or test applications! Principle of the Assay: alpha-GLA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-alpha-GLA antibody and an alpha-GLA-HRP conjugate. The assay sample and buffer are incubated together with alpha-GLA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha-GLA concentration since alpha-GLA from samples and alpha-GLA-HRP conjugate compete for the anti-alpha-GLA antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha-GLA from the sample, fewer sites are left to bind alpha-GLA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha-GLA concentration in each sample is interpolated from this standard curve.

Human

Quantitative Competitive

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

0.1 ng/mL