Human amyloid beta peptide 1-42, Abeta1-42 96T ELISA Kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Abeta1-42 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Abeta1-42 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Abeta1-42 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Abeta1-42 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human

0.312 ng/ml-20 ng/ml

Quantitative Sandwich

Serum, plasma, tissue homogenates, cerebrospinal fluid (csf)

< 0.078 ng/ml

Human amyloid beta peptide 1-42; Abeta1-42 ELISA Kit; Amyloid beta 42 (ABeta 42) ; amyloid beta peptide 1-42; Abeta1-42