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hBrachyury MAb (Cl 1 (25 ug)

品牌： R&D Systems

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产品介绍

产品信息

荧光素标记

Unconjugated

纯化方式

Protein A or G purified from cell culture supernatant

抗原名称

brachyury protein， Brachyury， MGC104817， Protein T， SAVA， T brachyury (mouse) homolog， T brachyury h

宿主

Rabbit IgG

免疫原

E. coli-derived recombinant human Brachyury
Met230-Met435
Accession # O15178

克隆号

1161B

Scientific Data

Immunocytochemistry

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Brachyury in BG01V Human Embryonic Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Human Brachyury Antibody Summary

Species Reactivity

Human

Specificity

Detects human Brachyury in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1161B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human Brachyury
Met230-Met435
Accession # O15178

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

Immunocytochemistry

背景

别名

brachyury protein,Brachyury,MGC104817,Protein T,SAVA,T brachyury (mouse) homolog,T brachyury homolog,T Brachyury Transcription Factor,T, brachyury homolog (mouse),T-Box Transcription Factor T,TBXT,TF,TFT

背景

Background: Brachyury

Brachyury is a 435 aa T-box family transcription factor. Human Brachyury shares 90% and 91% aa identity with mouse and rat Brachyury, respectively. It is required in the early determination and differentiation of mesoderm. Additionally, expression of Brachyury has been shown to be upregulated in a number of cancers, and in some cases, correlates with increased aggressiveness.

Entrez Gene IDs

6862 (Human)

Alternate Names

brachyury protein; Brachyury; MGC104817; Protein T; SAVA; T brachyury (mouse) homolog; T brachyury homolog; T Brachyury Transcription Factor; T, brachyury homolog (mouse); T-Box Transcription Factor T; TBXT; TF; TFT

研究领域

Related Research Areas

制备和贮存

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Brachyury in BG01V Human Embryonic Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 1:50 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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货号：

MAB20851-SP

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询价

25ug

100UG

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