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hClaudin-4 MAb (Cl 3 (25 ug)

hClaudin-4 MAb (Cl 3 (25 ug)

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品牌: R&D Systems
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来源宿主:
Mouse IgG2a
实验应用:
Flow Cytometry(2.5 µg/106 cells)
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
claudin 4, Claudin4, Claudin-4, CLDN4, Clostridium perfringens enterotoxin receptor 1, Clostridium p
宿主
Mouse IgG2a
免疫原
NS0 mouse myeloma cell line transfected with human Claudin-4
Met1-Val209
Accession # NP_001296
克隆号
382321

Scientific Data

Flow Cytometry Detection of Claudin-4 antibody in PC-3 Human Cell Line antibody by Flow Cytometry.View Larger

Detection of Claudin‑4 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB4219, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Human Claudin-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Claudin‑4. Stains human Claudin-4 transfectants but not irrelevant transfectants. Recognizes an epitope in the first extracellular loop (aa Met29-Arg81).
Source
Monoclonal Mouse IgG2A Clone # 382321
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-4
Met1-Val209
Accession # NP_001296
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Flow Cytometry(2.5 µg/106 cells)
背景
别名
claudin 4,Claudin4,Claudin-4,CLDN4,Clostridium perfringens enterotoxin receptor 1,Clostridium perfringens enterotoxin receptor,CPE-R,CPE-RCPETR,CPE-receptor,CPETR1,hCPE-R,WBSCR8,WBSCR8CPERCPETR1claudin-4,Williams-Beuren syndrome chromosomal region 8 protein
背景

Background: Claudin-4

Claudin-4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin-4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also up‑regulated in several carcinomas. Phosphorylation of Claudin-4 regulates paracellular epithelial permeability. Human Claudin-4 shares 83% aa sequence identity with mouse and rat Claudin-4.

Entrez Gene IDs
1364 (Human); 12740 (Mouse); 304407 (Rat)
Alternate Names
claudin 4; Claudin4; Claudin-4; CLDN4; Clostridium perfringens enterotoxin receptor 1; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-RCPETR; CPE-receptor; CPETR1; hCPE-R; WBSCR8; WBSCR8CPERCPETR1claudin-4; Williams-Beuren syndrome chromosomal region 8 protein
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Detection of Claudin‑4 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin‑4 Monoclonal Antibody (Catalog # MAB4219, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

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货号:
MAB4219-SP
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